Figure 4
Secondary structure Analysis using circular dichroism. (A–C) CD spectra for HomA, 40-fold dilution to buffer containing detergent ×4 and ×10 concentration of CMC, LDAO, CHAPS, and TWEEN 20, respectively. (D,E) CD spectra for HomA, 40-fold dilution to a buffer containing 0.25 mM, 0.5 mM, and 1 mM DMPC and DOPC lipids, respectively. (F–H) CD spectra for HomB, 40-fold dilution to buffer containing detergent ×4 and ×10 concentration of CMC, LDAO, CHAPS, and TWEEN 20, respectively. (I,J) CD spectra for HomB, 40-fold dilution to a buffer containing 0.25 mM, 0.5 mM, and 1 mM DMPC and DOPC lipids, respectively.
