Figure 6
Effect of detergents and lipids on HomA and HomB folding. (A–C) Tryptophan fluorescence spectra of denatured HomA and 40 folds diluted in buffer containing CHAPS, LDAO, and TWEEN 20 detergents, respectively, ×4 and ×10 concentration of CMC. (D,E) Tryptophan fluorescence spectra of denatured HomA and 40 folds diluted in buffer containing DMPC and DOPC (0.25 mM and 1 mM), respectively. (F–H) Tryptophan fluorescence spectra of denatured HomB and 40 folds diluted in buffer containing CHAPS, LDAO, and TWEEN 20 detergents, respectively, ×4 and ×10 concentration of CMC. (I,J) Tryptophan fluorescence spectra of denatured HomB and 40 folds diluted in buffer containing DMPC and DOPC (0.25 mM and 1 mM), respectively.
