Figure 4
RNAseq of SF-295 and A549 cells treated with 50 nM TTI-3066 for 6 h or 24 h. Data is expressed as log10(treated–untreated). All heatmaps were generated with |log10(treated–untreated)|> 2, with 6 h data represented as the outside of the ring and 24 h data as the inside of the ring. Heatmaps of significantly altered genes in SF-295 (A) and A549 (B) are presented. Gene enrichment analysis for PANTHER pathways are included for SF-295 (C) and A549 (D) at 6 h and 24 h. Reactome and GO pathway analysis are included in supplemental tables. Principal components plots for 6 h (Supplementary Fig. 3A) and 24 h (Supplementary Fig. 3C) are included. At 6 h (Supplementary Fig. 3B) and 24 h (Supplementary Fig. 3D), some genes were differentially regulated similarly between SF-295 and A549. GO biological process top hits at 24 h for SF-295 (E) and A549 (F) are shown, with further GO and Reactome pathway analysis included in Supplementary Table 4.
