Figure 6
Schweinfurthins affect the hedgehog pathway. (A) Schematic of the hedgehog pathway. (B) Heatmap of gene expression for selected Hedgehog-related genes at 24 h. (C) Western blot of hedgehog pathway genes shows dose and time dependence of hedgehog protein response. (D) Compound response was determined via 48 h MTT assay in A549 in the presence or absence of 10 µM cyclopamine, a selective Hedgehog pathway inhibitor. (E-G) Hedgehog pathway related gene expression changes for GLI1 (E), PTCH2 (F), and SUFU (G) were investigated via qRT-PCR. Graphs represent fold change with vehicle treatment = 1 following two-way ANOVA with Bonferroni’s correction. GAPDH was used as an internal control gene. Relative gene expression was calculated using the 2−ΔCt method.
