Figure 5

E-protein mediated inflammasome activation is more pronounced in JAK^V617F human iPSC-derived macrophages. (A) Quantification of LDH release into cell media in Control and JAK2^V617F hPSC-derived macrophages, transduced with control and E-protein lentiviruses for 72 h and then primed with 20 ng/ml LPS for 3 h and treated with poly(I:C) for 16 h. LDH release was assessed via LDH kit. Data are presented as mean ± SD, which were analyzed by one-way ANOVA coupled with Tukey’s test for multiple comparisons. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05. (B) Quantification of IL-1β secretion into cell media in Control and JAK2^V617F hPSC-derived macrophages, transduced with control and E-protein lentiviruses for 72 h and then primed with 20 ng/ml LPS for 3 h and treated with poly(I:C) for 16 h. IL-1β secretion was assessed via IL-1β ELISA. Data are presented as mean ± SD, which were analyzed by one-way ANOVA coupled with Tukey’s test for multiple comparisons. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05. (C) Quantification of TNF-α secretion into cell media in Control and JAK2^V617F hPSC-derived macrophages, transduced with control and E-protein lentiviruses for 72 h and then primed with 20 ng/ml LPS for 3 h and treated with poly(I:C) for 16 h. TNF-α secretion was assessed via TNF-α ELISA. Data are presented as mean ± SD, which were analyzed by one-way ANOVA coupled with Tukey’s test for multiple comparisons. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05.