Table 1 Elemental cell surface composition determined by XPS for the wild-type, the ∆mreB and the ∆tagO mutants grown in LB medium supplemented or not with 25 mM Mg2+.

From: Magnesium rescues the morphology of Bacillus subtilis mreB mutants through its inhibitory effect on peptidoglycan hydrolases

 

C

O

N

P

Mg

K

Na

Ca

wt

65,7

25,3

4,9

2,4

0,3

0,1

1,1

0,1

66,0

25,1

4,6

2,2

0,5

0,1

1,3

bdl

wt + Mg2+

72,7

21,2

2,7

1,7

0,9

bdl

0,2

0,6

64,6

27,2

4,4

2,0

0,9

0,2

0,6

0,1

ΔmreB

62,1

26,7

6,0

2,4

0,3

0,9

1,5

0,1

66,7

23,9

5,7

2,0

0,3

0,2

1,1

0,1

ΔmreB + Mg2+

59,0

31,0

5,2

2,9

1,3

bdl

0,6

0,1

63,1

27,5

5,8

2,0

0,8

0,2

0,5

bdl

ΔtagO + Mg2+

66,3

23,5

8,6

0,2

0,9

bdl

0,4

bdl

58,8

29,0

9,8

0,2

1,3

bdl

0,4

bdl

  1. Note that the ΔtagO null mutant only grew in liquid LB in the presence of high Mg2+ (Supplementary Fig. S9a) and thus XPS analysis could only be performed at high Mg2+. Results are expressed as molar fractions (in %), excluding hydrogen. Results of two independent sets of experiments are presented. C, carbon; O, oxygen; N, nitrogen; P, phosphorus; Na, sodium; Mg, magnesium; Ca, calcium and K, potassium. bdl = below detection limit.
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