Figure 2 | Scientific Reports

Figure 2

From: Endothelial-derived extracellular microRNA-92a promotes arterial stiffness by regulating phenotype changes of vascular smooth muscle cells

Figure 2

Ang II induces miR-92a and EC dysfunction. (A) miRNA qPCR analysis of miR-92a level in HUVECs treated with various concentrations of Ang II for 24 h. (B) Relative miR-92a level in HUVECs treated with Ang II (200 nM) for different times. (C, D) HUVECs were treated with Ang II (200 nM) or PBS (Ctrl) for 24 h. mRNA and protein levels of KLF2, KLF4, eNOS, and ET-1 were measured by qPCR and western blot, respectively. Data are mean ± SEM from 3 independent experiments in (A–C). Parametric data were analyzed by the one-way ANOVA between multiple groups (A, B) and by the two-tailed Student-t test with Welch correction between 2 indicated groups (C). *p < 0.05, **p < 0.01, ***p < 0.001. The eNOS, KLF4, KLF2, ET-1 and β-actin bands were cropped from full gels (Supplementary Fig. S1a).

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