Figure 3
From: Suppression of lipopolysaccharide-induced corneal opacity by hepatocyte growth factor

Topical administration of HGF prevents keratitis-induced corneal opacity. Keratitis was induced by corneal injection of LPS on day 1 and day 4 followed by topical application of 2 µl of 0.1% HGF thrice a day for five days. Bright-field images were captured on days 4, 7, and 10 following the first injection. (A) Schematic of experimental design, (B) Representative bright-field images (left) cumulative bar chart (right) depicting the size of corneal opacity in mice treated with the indicated treatment. Bright-field images were converted into a binary mode (black and white) using ImageJ software with black corresponding to high opacity areas. Results were expressed as percent restoration of corneal transparency on day 10 compared to day 4. Representative data from experiments consisting of 6 animals per treatment group. Data presented are mean ± SEM. **p < 0.01.