Table 2 Optimization of PMS-phage assay on MAP-spiked milk samples represented by the number of lysed plaques developed on MB 7H10 agar and the positivity status of DNA extracted from lysed plaques via qPCR IS900 analysis.
From: A rapid phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis in milk
Dilutions/concentrations | Number of plaques in milk | qPCR IS900 |
|---|---|---|
10−1/106 | Totally lysed | – |
10−2/105 | Totally lysed | – |
10−3/104 | Many | Positive |
10−4/103 | 31 | Positive |
10−5/102 | 30 | Positive |
10−6/101 | 0 | – |
