Table 5 Standardization of qPCR IS900 analysis and threshold cycles (TC) corresponding to numbers of MAP copies in each reaction.
From: A rapid phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis in milk
Copy numbers of dna template (copies/μL) | Threshold cycle (TC) in qPCR IS900 |
|---|---|
107 | 18.5 |
106 | 22.3 |
105 | 26 |
104 | 29.5 |
103 | 33 |
102 | 36.3 |
101 | 37.4 |
1 | 0 |
0.1 | 0 |
0.01 | 0 |
