Figure 1
From: Drosophila septin interacting protein 1 regulates neurogenesis in the early developing larval brain
Neurogenesis of vPNs is significantly impaired when the P111477 mutation is induced at early but not late larval stages. (a) An example of a MARCM neuroblast (NB) clone is used to reveal the morphology of vPNs in the adult brain; using GAL4-MZ699. Axons (arrowhead) were primarily projected to the lateral horn, while dendrites (arrow) were found within the AL, and cell bodies (dashed circle) were distributed ventral to the AL. (b) Schematic drawings show predicted outcomes of MARCM clones induced at different developmental stages: high, medium and low numbers of neurons should be respectively observed when MARCM clones are induced at early, middle and late developmental stages. (c) Numbers of labeled vPNs were shown for wild-type (d–g) and P111477 mutants (h–k) when MARCM clones were induced at different larval stages. (d–k) Gradually reduced numbers of labeled vPNs (within the dashed circle) were observed in wild-type samples (d–g) when MARCM clones were induced at 24 h after larval hatching (NHL-24 h ALH), 48 h ALH, 72 h ALH and 96 h ALH. vPN neurogenesis was significantly impaired in the P111477 mutant samples (h–k) induced at early but not late larval stages. The genotypes shown in all figures are summarized in Supplemental table 1. Neuropils were revealed by the Bruchpilot (Brp) staining (blue). Scale bar: 10 μm.
