Figure 2
From: Drosophila septin interacting protein 1 regulates neurogenesis in the early developing larval brain
Neurogenesis is generally compromised in various brain regions when the Nuwa mutation is induced at the early but not late larval stages. (a) Three groups of neurons were used to examine the developmental stage-dependent requirement for Nuwa, including neurons in the subesophageal zone (SEZs; panels b–e), ventral olfactory interneurons in the AL (vLNs; panels f–i) and neurons in the ventrolateral protocerebrum (VLPs; panels J-M). (b–m) All three groups of neurons displayed severe neurogenesis defects with the P102523 (Nuwa) mutation when MARCM clones were induced at NHL-24 h ALH (panels b, d, f, h, j, l), whereas no obvious neurogenesis defects were observed in neurons with the Nuwa mutation when MARCM clones were induced at 72 h ALH (panels c, e, g, i, k, m). Neuropils were revealed by the Brp staining (blue), and background neurons are indicated by arrowheads. Scale bar: 10 μm.
