Figure 3

Embryonic-born adPNs are produced normally but postembryonic-born adPNs fail to appear when the Nuwa mutation is induced at the embryonic stage. (a) Schematic drawings show a predicted outcome for a twin-spot MARCM clone when it is induced at the embryonic stage (upper panel); the known cell numbers and subtypes of embryonic- and larval-born adPNs labeled by GAL4-GH146 are shown (bottom panel). (b, c) Examples of wild-type and Nuwa mutant twin-spot MARCM clones induced at the embryonic stage. In the wild-type sample (panel b), a VM3a adPN (an embryonic-born adPN in magenta) was associated with around 35 adPNs (containing both embryonic- and larval-born adPNs in green); in the P¹⁰²⁵²³ (Nuwa) mutant (panel c), a VM3a wild-type adPN (magenta) was associated with three Nuwa mutant adPNs (green, marked by asterisks). Dendrites of the three green Nuwa mutant adPNs were only observed in DM3, VM3 and DL4 [but not DL1 (indicated by dashed arrow)] glomeruli of the AL, indicating that they belong to the last three types of embryonic-born adPNs and further suggesting that no larval-born adPNs were generated. Neuropils were revealed by Brp staining (blue), and background neurons are indicated by arrowheads. Scale bar: 10 μm.