Figure 5
From: Rational design of a helical peptide inhibitor targeting c-Myb–KIX interaction
Surface plasmon resonance (SPR) competitive binding assay. The wild-type Myb32 was immobilized on a sensor chip, and a mixture of KIX and various concentrations of an inhibitor was injected. (a) The maximum level of SPR response was plotted against the concentration of the wild-type Myb32 (black) and the designed RRR inhibitor (red). The inhibitor concentration is plotted in a logarithmic scale. The continuous lines were obtained by fitting to Eq. (5). The experiments were performed in triplicate, and the mean and standard error are shown. (b) The IC50 values. The errors are standard errors.
