Table 1 Thermodynamic parameters for the KIX binding of Myb32 mutants.
From: Rational design of a helical peptide inhibitor targeting c-Myb–KIX interaction
Myb32 | Kd (μM) | N | ΔH (kcal mol–1) | –TΔS (kcal mol–1) | ΔG (kcal mol–1) |
|---|---|---|---|---|---|
WT | 0.22 ± 0.01 | 0.97 ± 0.01 | –6.49 ± 0.05 | –2.74 ± 0.04 | –9.23 ± 0.04 |
K291R | 0.21 ± 0.01 | 0.97 ± 0.01 | –6.46 ± 0.04 | –2.80 ± 0.01 | –9.27 ± 0.04 |
E292L | 0.48 ± 0.03 | 0.92 ± 0.02 | –5.45 ± 0.03 | –3.32 ± 0.01 | –8.77 ± 0.04 |
K293R | 0.22 ± 0.01 | 0.94 ± 0.01 | –7.03 ± 0.01 | –2.21 ± 0.02 | –9.23 ± 0.01 |
K296R | 0.16 ± 0.01 | 0.96 ± 0.02 | –6.3 ± 0.1 | –3.2 ± 0.2 | –9.44 ± 0.04 |
K291R/K293R | 0.14 ± 0.01 | 0.84 ± 0.02 | –7.31 ± 0.04 | –2.21 ± 0.03 | –9.51 ± 0.01 |
K291R/K296R | 0.12 ± 0.01 | 0.90 ± 0.01 | –7.56 ± 0.03 | –2.05 ± 0.03 | –9.62 ± 0.05 |
K293R/K296R | 0.13 ± 0.01 | 0.96 ± 0.01 | –7.31 ± 0.04 | –2.23 ± 0.08 | –9.54 ± 0.04 |
RRR | 0.08 ± 0.01 | 0.99 ± 0.01 | –7.57 ± 0.03 | –2.27 ± 0.07 | –9.84 ± 0.09 |
WT (SPR)a | 0.51 ± 0.02 | 0.99 ± 0.02 | –13.79 ± 0.03 | 5.07 ± 0.05 | –8.72 ± 0.02 |
RRR (SPR) | 0.26 ± 0.01 | 0.99 ± 0.01 | –14.80 ± 0.03 | 5.67 ± 0.02 | –9.14 ± 0.01 |
