Figure 3 | Scientific Reports

Figure 3

From: Endoplasmic reticulum stress and the unfolded protein response in skeletal muscle of subjects suffering from peritoneal sepsis

Figure 3

Indicators of apoptosis in skeletal muscle. TUNEL staining and semiquantitative assessment of positive cells (a) in human skeletal muscle in sepsis compared to the other groups (*p < 0.05, χ2-test). Arrows indicate TUNEL positive cells (brownish coloring), which are representative for cells undergoing apoptosis. Gene expression analysis for apoptotic marker genes BAX and CHOP, which are related to UPR activation, in human (b,d) and murine (c,e) skeletal muscle. Data are given as normalized log2-fold changes compared to the median of the respective control groups as reference. Plots for human data represent n = 10 of matched subjects per group (Suppl. Table 2), plots for animal data represent n = 5–6 animals per group. BAX/Bax bcl-2-associated X protein, CD control diet, CHOP/Chop C/EBP homologous protein, HFD high-fat diet, IR insulin resistance, TUNEL TdT-mediated dUTP-biotin nick end labeling; *p < 0.05.

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