Figure 3
From: KIAA0319 influences cilia length, cell migration and mechanical cell–substrate interaction
Analysis of mechanical activity of RPE1 WT and Ex6KO cells during migration on an ERISM micro-cavity. (A) Phase contrast (upper row) and ERISM micro-cavity displacement maps (lower row) of WT (left) and Ex6KO (right) cells. (B) Comparison of the surface area covered by WT (n = 36) and Ex6KO (n = 36) cells types. (C) Comparison of mean speed of WT (n = 29) and Ex6KO (n = 24) cells. (D) Comparison of mean indented volume of WT (n = 29) and Ex6KO (n = 24) cells. Only cells with free movement for ≥ 2 h were included in analysis of speed and indented volume. Plots in (B,D,E) show all measured data points and the mean (line). Groups were compared using the Student’s t-test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Origin 2018 version 95E (https://www.originlab.com/2018) was used for data plotting and significance testing. Scale bar, 50 μm.
