Figure 4
From: KIAA0319 influences cilia length, cell migration and mechanical cell–substrate interaction
Phenotype recovery through KIAA0319 rescue. (A) Western blot confirming the presence of a fusion protein (140 KDa) following transfection with a full length KIAA0319 construct fused to a GFP tag. Full-length blots are presented in Supplementary Fig. S3. (B) Comparison of area covered by RPE1 WT, WT K-GFP, Ex6KO and Ex6KO K-GFP cells attached to ERISM micro-cavity. (WT: n = 16, WT K-GFP: n = 20, Ex6KO: n = 23, Ex6KO KGFP: n = 17). (C) Comparison of mean mechanical activity of RPE1 WT, WT K-GFP, Ex6KO and Ex6KO K-GFP cells during migration on ERISM micro-cavity. Only cells with free movement for > 4 h were included in the analysis. Plots in B and C show measured data points and the mean (line). (WT: n = 16, WT K-GFP: n = 19, Ex6KO: n = 24, Ex6KO K-GFP: n = 16) Groups were compared using the Student’s t-test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). Origin 2018 version 95E (https://www.originlab.com/2018) was used for data plotting and significance testing.
