Figure 5
From: KIAA0319 influences cilia length, cell migration and mechanical cell–substrate interaction
RPE1 KIAA0319 WT and Ex6KO cells use different modes of force exertion. (A) Phase contrast images (upper row), Fourier-filtered ERISM displacement maps (middle row) and epi-fluorescence images (lower row, red: actin, green: vinculin, blue: nuclear DNA) of a RPE1 WT cell (left column) and an Ex6KO cell (right column). The insets (i) in the Fourier-filtered ERISM map and the epi-fluorescence image of the WT cell show magnifications of protrusions linked to actin dots. Arrows indicate positions of actin-rich cell protrusions that are counterbalanced by pulling at vinculin-rich positions marked with circles. The insets (ii,iii) show magnifications of vinculin-rich cell-substrate contacts (focal adhesions) for the WT and Ex6KO cell, respectively. (B) Temporal evolution of the indentation force applied by different actin dots of the WT cell shown in (A). (C,D) Temporal evolution of the contraction force applied by different focal adhesions of the WT and Ex6KO cell shown in A, respectively. (E) Comparison of the fraction of WT and Ex6KO cells forming actin dot protrusions. Each data point represents the mean value of an independent experiment investigating n = 5 (80%), 3 (67%) and 4 (50%) WT and n = 5 (0%), 6 (0%) and 9 (33%) Ex6KO cells, respectively. The lines depict the means. Groups were compared using the Student’s t-test (*p ≤ 0.05). Origin 2018 version 95E (https://www.originlab.com/2018) was used for data plotting and significance testing. All scale bars: 20 μm.
