Figure 2
From: Annexin A6 and NPC1 regulate LDL-inducible cell migration and distribution of focal adhesions
AnxA6 overexpression and NPC1 inhibition compromise LDL-inducible cell migration. (a,b) Wound healing assays with A431-WT and A431-A6 cells grown in 10% LPDS-containing media together with LDL (50 µg/ml), U18666A (4 µg/ml), or both using the IncuCyte were performed. Representative images at t = 0 and 16 h are shown (a). RWD (%) and cell velocity (µm/h) from 3 independent experiments with triplicate samples (mean ± SD) was calculated (b). (c,d) A431-WT and A431-A6 cells were grown in LPDS-containing media ± LDL (50 µg/ml) and EGF (10 ng/ml) as indicated and cell migration was monitored. Representative images at t = 0 and 14 h are shown (c). RWD (%) and cell velocity (µm/h) from 3 independent experiments with triplicate samples (mean ± SD) was calculated (d). For each individual experiment shown in (b,d), the mean is indicated by dot points in each bar graph. *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA with Tukey’s post-hoc test.
