Figure 3
From: Annexin A6 and NPC1 regulate LDL-inducible cell migration and distribution of focal adhesions
NPC1 inhibition and AnxA6 overexpression inhibit LDL-inducible A431 cell invasion. (a) Transwell migration and invasion of A431-WT cells treated ± U18666A (4 µg/ml). Representative images of migrating and invading cells from five fields per condition of a representative experiment (n = 2) were quantified and relative invasion (%) was calculated (mean ± SD). Individual data points from the five fields are indicated by dot points in each bar graph. (b) A431-WT and A431-A6 cells on three-dimensional matrices of rat tail collagen were grown in 10% LPDS-containing media ± LDL (50 μg/ml) fixed, and stained with hematoxylin and eosin. The relative invasion (%) was calculated as the percentage of cells that invaded beyond ≈30 μm as a percentage of total cells in the assay. Representative images from 2 independent experiments are shown. The mean ± SD from 4–6 images per condition is given. Individual data points are indicated by dot points in each bar graph. *p < 0.05, **p < 0.01, ***p < 0.001; Mann–Whitney two-tailed test (a), and two-way ANOVA with Tukey’s post-hoc test (b).
