Figure 6
From: Annexin A6 and NPC1 regulate LDL-inducible cell migration and distribution of focal adhesions
Distribution of FA marker vinculin in CHO M12 and CHO M12-A6ko cells. (a) CHO-WT, CHO M12 and CHO M12-A6ko cells ectopically expressing EGFP-vinculin were serum-stimulated (20% FCS) and stained for phosphorylated FAK (pY861FAK, red) as indicated. The merged images and enlarged regions of interest are shown. Arrows indicate colocalization of EGFP-vinculin and pY861FAK in CHO-WT cells. Arrowheads point at pY861FAK in CHO M12 or EGFP-vinculin staining in CHO M12-A6ko cells, respectively. Bar is 10 µm and 5 µm for enlarged insets. (b) Colocalization of EGFR-vinculin and pY891FAK was quantified (Pearson’s R value; 20–30 cells per condition and cell line were quantified. (c) Focal adhesions (> 0.25 µm2) per cell (left panel) and the ratio of focal adhesions at the cell edge vs. cell body (right panel) was quantified. 7–13 cells per condition and cell line were counted. The mean ± SD is given. *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA with Tukey’s post-hoc test.
