Figure 7
From: Annexin A6 and NPC1 regulate LDL-inducible cell migration and distribution of focal adhesions
LDL stimulates FA formation at the cell edges of WT, but not NPC1-deficient cells. (a) CHO-WT, CHO M12 and CHO M12-A6ko cells grown in 10% LPDS-containing media ± LDL (50 μg/ml) were immunolabeled with anti-pY861FAK. Arrowheads point at macroscopic pY861FAK-positive structures at the cell edge in CHO-WT and CHO M12-A6ko cells. Bar is 20 µm. (b) Focal adhesions (> 0.25 µm2) per cell (left panel) and the ratio of focal adhesions at the cell edge vs. cell body (right panel) were quantified. 16–21 cells per condition and cell line were counted. The mean ± SD is given. *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA with Tukey’s post-hoc test.
