Figure 8
From: Annexin A6 and NPC1 regulate LDL-inducible cell migration and distribution of focal adhesions
AnxA6-deficient NPC1 mutant cells display increased association of cholesterol with FA structures. (a) CHO M12 and CHO M12-A6ko cells ectopically expressing the cholesterol biosensor mCherry-D4H (red) were serum-stimulated (20% FCS) and stained for phosphorylated FAK (pY861FAK, green) as indicated. The merged images are shown. In CHO M12 cells, different distributions of D4H and pY861-FAK are common (see line profiles A–B, C–D, in b, top panel), respectively. In CHO M12-A6ko cells, D4H and pY861FAK often colocalize at cell edges (see line profiles E–F, G–H in b, bottom panel). Bar is 10 µm. (b) The line profiles of fluorescence intensities (a.u.) of pY861FAK (green) and mCherry–D4H (red) in CHO M12 (A–B, C–D, 0–20 μm, top panel) and CHO M12-A6ko cells (E–F, 0–20 μm and G–H, 0–25 μm, bottom panel) are shown.
