Figure 3

LC3 immunostained SMG sections showing positive nuclear and cytoplasmic reaction in intralobular ducts and GCD (arrows) in control group (A,B), while diabetic group showed strong positive cytoplasmic and nuclear reaction in both acini and ducts (arrows) (C,D). GA treated group (E,F) revealed a positive reaction in ducts only. (LC3, × 10, inserts × 20). Quantitative analysis of LC3 immunostaining (%area) (G) and fold change of LC3 gene expression (H). Quantitative analysis of ELISA results, LC3 II expression (I). Data were expressed as mean ± SE. P < 0.05 was considered significant. ****Significant compared to control group at P < 0.0001, ^###Significant compared to diabetic group at P < 0.001and ^####Significant compared to diabetic group at P < 0.0001. P62 immunostained SMG sections showing both cytoplasmic and nuclear positive reaction especially in the ducts in DM group (K). While, little mild cytoplasmic reaction and nuclear ductal reaction in both control (J) and GA group (L) (arrows) (P62 IHC, × 20). (M) Quantitative analysis of P62 immunostaining (%area). (N) Effect of GA treatment on Beclin-1expression. Data were expressed as mean ± SE. P < 0.05 was considered significant. ****Significant compared to control group at P < 0.0001, ^####Significant compared to diabetic group at P < 0.0001.