Figure 2 | Scientific Reports

Figure 2

From: Repurposing the Pathogen Box compounds for identification of potent anti-malarials against blood stages of Plasmodium falciparum with PfUCHL3 inhibitory activity

Figure 2

Expression, purification and deubiquitinating activity of PfUCHL3 and HsUCHL3: (a) PfUCHL3 and (b) HsUCHL3 were expressed in BL21 (λDE3) and purified using Ni-affinity chromatography, UI-uninduced, I-induced, Ub-unbound, W-wash. The proteins were further purified by gel filtration chromatography using AKTA prime. (c) The FPLC profile denotes a single peak for both the enzymes. Both (d) PfUCHL3 and (e) HsUCHL3 were purified to homogeneity as determined by SDS-PAGE analysis. (f) The catalytic activities of both the enzymes were assessed by measuring the change in fluorescence intensity, substrate and enzyme alone were taken as negative control. Uncropped images of commassie stained SDS gels are provided in Supplementary Fig. S1.

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