Figure 8 | Scientific Reports

Figure 8

From: Repurposing the Pathogen Box compounds for identification of potent anti-malarials against blood stages of Plasmodium falciparum with PfUCHL3 inhibitory activity

Figure 8

Effect of MMV676603 and MMV688704 on the growth and morphology of intraerythrocytic stage of P. falciparum, and assessment of their speed of action: synchronized P. falciparum 3D7 culture was treated with leads (MMV676603 and MMV688704) and their effect on parasite phenotype and growth was analyzed by (a) Giemsa stained blood smear micrographs at 0, 8, 16, 24, 36, 48 and 56 h (two representative images of each set). The speed of action of both the compounds (MMV676603 and MMV688704) was evaluated for one complete maturation cycle [starting from ring (0 h), trophozoite (16–24 h) to schizont (36 h)]. Both the compounds were considered as slow inhibitors as they hinder the parasite development at late stages (trophozoite and schizont). (b) The in-vitro reduction in parasitemia level was quantified and illustrated as a graph, indicating the parasitemia level as a function of parasite life cycle progression (time). Data are represented as Mean ± S.D.

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