Figure 9 | Scientific Reports

Figure 9

From: Repurposing the Pathogen Box compounds for identification of potent anti-malarials against blood stages of Plasmodium falciparum with PfUCHL3 inhibitory activity

Figure 9

Effect of leads on ubiquitination levels: asynchronous P. falciparum culture was treated with vehicle control (Lane 1), Chloroquine (Lane 2) and identified compounds (MMV676603 (Lane 3), MMV688704 (Lane 4)). Following treatment, parasites were lysed and equal amount of parasite lysate was subjected to SDSPAGE and immunoblotting was carried out with ubiquitin antibody for ubiquitination levels. β-actin was kept as loading control. The experiment was performed atleast thrice with a respresentative image shown as figure. Full blots are provided in Supplementary Fig. S2.

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