Figure 4
Chromatographic data on the photoradiosynthesis of 89Zr-labeled proteins. (a) SK-10 size-exclusion elution profiles of 89ZrDFO-PEG3-ArN3 (dashed-red), the photolyzed solution of 89ZrDFO-PEG3-ArN3 in the absence of protein (green), the crude reaction containing the 89ZrDFO-PEG3-azepin-trastuzumab product (black), and the measured protein content in the crude reaction mixture (orange). (b) Experimental data on the radiochemical conversion (RCC/%; black; measured after the photolysis step) and the decay-corrected isolated radiochemical yields (RCY/%; blue) for the automated photoradiosynthesis of 89ZrDFO-PEG3-azepin-trastuzumab using ALISI with different initial concentrations of trastuzumab. (c) SEC chromatograms of the isolated 89ZrDFO-PEG3-azepin-trastuzumab product (produced by full automation using ALISI) showing the radioactive profile (blue) and the protein elution profile measured by electronic absorption at 280 nm (orange).
