Figure 3
From: A mutation in transmembrane protein 135 impairs lipid metabolism in mouse eyecups
RNA-Seq analysis of 2.5-month-old homozygous Tmem135 mutant eyecups compared to heterozygous Tmem135 mutant eyecups. (A) Volcano plot highlighting differentially-expressed genes with fold changes greater than 2 that reach statistical significance (p < 0.05) between Tmem135FUN025/FUN025 (FUN025/FUN025) and Tmem135FUN025/+ (FUN025/+) eyecups. Genes that are upregulated and downregulated are highlighted in magenta and cyan, respectively. Heatmaps of differentially-expressed genes in the leading edge with greater than log2 fold changes between Tmem135FUN025/FUN025 and Tmem135FUN025/+ eyecups involved in (B) cholesterol metabolism, (C) PPAR signalling, and (D) complement and coagulation cascades which were identified using gene set enrichment analysis. These genes were unchanged between Tmem135FUN025/+ and WT eyecups.
