Figure 3
Volcano plot summarizing the results obtained from the differential expression analysis between critical and non-critical patients. X-axis represents the log2-transformed fold-change (FC). Y-axis shows the minus-log10-transformed p value associated to the protein in the comparison. Positive log2-fold-changes indicate over-expression in critical patients while negative log2-fold-changes represent over-expression in non-critical patients. Results were derived from a linear mixed-effects model fitted to each protein independently that included peptide and patient as random effects. Digestion batch, evolution group (critical/non-critical), blood extraction day and the interaction of the two latter were modelled as fixed effects. Comparisons were performed by averaging time point estimations within each evolution group. Statistical significance was assessed using a Wald test derived from the models. (Figure created using R version 3.6.0, https://www.R-project.org/).
