Figure 4
From: Innate immune deficiencies are associated with severity and poor prognosis in patients with COVID-19
Anti-microbial functions of circulating phagocytes are impaired in severe COVID-19. (A,B) Measurement of phagocytosis capacity by phRodo-conjugated Zymosan particles uptake in (A) neutrophils and (B) monocytes. (C,D) Measurement of (C) oxidative burst in response to medium, Tumor Necrosis Factor alpha (TNF), lipopolysaccharide (LPS), TLR8 ligand CLO97, and (D) priming of formyl-methionine-leucine-phenylalanine (fMLF)-induced burst by TNF, LPS and CLO-97 in neutrophils. (E) Measurement of neutrophils NETosis capacity in response to medium, phorbol myristate acetate (PMA), peptidoglycan (PGN), TNF and fMLF, LPS and fMLF. Intergroup comparison by Mann–Whitney U test between healthy controls and ICU or non-ICU patients, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05., between non-ICU and ICU patients, ##P < 0.01, #P < .05. All boxplots whiskers represent 10th and 90th percentiles.
