Figure 2

Recombinant ZIKV envelope vaccine elicited Env_M- and Env_Z -specific IgG induction. (A) Experimental strategies. C57BL/6 mice (n = 5) were immunized with ZIKV Env_M, Env_Z or PBS at weeks 0, 2 and 6. To measured humoral immune response, blood samples were collected at weeks 0, 4 and 7. (B) Serum samples were diluted 1:50,000 and measured for OD 450 values by using ZIKV Envelope protein coated ELISA kit (Alpha diagnostic international). Calculations of ZIKV ENV specific IgG antibody activity units. (C) Serum samples were diluted 1:100 measured for OD 450 values by using indirect ELISA. (D) Ratios of IgG2c to IgG1 antibodies in Env_M, Env_Z or PBS immunized mice. The data were represented the mean ± SD of five mice per group. Each sample was assayed in duplicates. Statistical analysis was performed comparing immunized group with PBS injected animals and represented *p < 0.05, **p < 0.01, ***p < 0.001.