Figure 4
Quantification of IFN-γ secretion among Ag-specific splenocytes following recombinant vaccination was analyzed by ELISPOT. C57BL/6 mice (n = 5) were inoculated with ZIKV Env_M, Env_Z or PBS at weeks 0, 2 and 6. Mice were euthanized 7 (Day 7; A,B, Day 17; C,D) or 3 (Day 45; E,F) days after the last immunization. Splenocytes were isolated and pooled from five mice per each group. Splenocytes were stimulated with same dose of (1 μg/ml) Env_M, Env_Z or PBS. Env_M and Env_Z specific IFN-γ responses were identified by ELISPOT assay at 48 h after stimulation. (A,C,E) Representative ELISPOT results. (B,D,F) Summary of the ELISPOT results. The data were represented the mean ± SD of five mice per group. Each sample was assayed in triplicates. Statistical analysis was performed comparing immunized group with PBS injected animals and represented *p < 0.05, **p < 0.01, ***p < 0.001.
