Figure 5
Effect of repeated Env_M, Env_Z immunization on effector cytokines production in splenocytes. C57BL/6 mice (n = 5) were immunized with ZIKV Env_M, Env_Z or PBS at weeks 0, 2. Mice were euthanized 7 (Day 7; A–C, Day 17; D–F) days after the last immunization. Splenocytes were isolated and pooled from five mice per each group. Splenocytes were stimulated with same dose of (1 μg/ml) Env_M, Env_Z or PBS. The supernatants harvested after 48 h of incubation and used to measure the concentrations of IFN-γ, TNF-α and IL-12 using ELISA. The data were represented the mean ± SD of five mice per group. Each sample was assayed in triplicates. Statistical analysis was performed comparing immunized group with PBS injected animals and represented *p < 0.05, **p < 0.01, ***p < 0.001.
