Figure 6
Level of vaccine specific maternal antibody in neonates’ circulation. C57BL/6 mice (n = 5) were immunized with ZIKV Env_M, Env_Z, or PBS at weeks 0, 2 and 6. Immunized mice bred as homozygous breeding pairs. To measured Env_M (A) or Env_Z (B) specific IgG antibodies, blood samples were collected on Day 2. Serum samples were twofold serially diluted from 1:100 to 1:3200 and measured for OD 450 values by using ELISA. The data were represented the mean ± SD of five mice per group. Each sample was assayed in duplicates. Statistical analysis was performed comparing immunized group with PBS injected animals and represented *p < 0.05, **p < 0.01, ***p < 0.001.
