Figure 7
Evaluation of protective effect of recombinant vaccine against ZIKV or DENV challenge. Female C57BL/6 mice (n = 5) were administrated with ZIKV Env_M, Env_Z, or PBS at the week of 0, 2, & 6, and were bred with their homozygous males (C57BL/6). Two-day-old neonatal mice were inoculated with PBS (A,B), 106.8 TCID50/mouse of MR766 strain (C,D), 106.3 TCID50/mouse of PRVABC59 strain (E,F) or 106 TCID50/mouse of DENV-2 strain (G,H). The survival rate and body weight change of neonatal mice (n = 5 per group) was monitored until 3 weeks post infection. The body weight data were represented the mean ± SD of five mice per group (A,C,E,G). The survival rate were represented the Kaplan–Meier survival curves (B,D,F,H). Statistical analysis was performed comparing immunized group with PBS injected animals and represented *p < 0.05, **p < 0.01, ***p < 0.001.
