Figure 4
Blockade of HSP70 enhances the inhibitory effect of an L-type \(\hbox {Ca}^{2+}\) channel blocker in aorta stimulated with phenylephrine. Aortic rings were challenged with phenylephrine (\(10\,\upmu \hbox {mol/l}\)) in 0[\(\hbox {Ca}^{2+}\)] Krebs’ solution in the presence of vehicle (CTL) or (A) verapamil (1 and 3 \(\upmu \)mol/l, DMSO diluted) or (B) VER155008 (\(10\,\upmu \hbox {mol/l}\), DMSO diluted) as well as the combination of verapamil and VER155008. Inhibitors were added after IP3r-mediated \(\hbox {Ca}^{2+}\) release. The concentration of \(\hbox {Ca}^{2+}\) was restored after 10 min, and the force developed was evaluated for 15 min. (C) Area under the curve and (D) \(\hbox {E}_{\mathrm{max}}\). Panels (C,D) use the same color scheme as panels (A,B). Data are expressed as mean ± SEM. n = 9 for CTL and VER and n = 5 for all other groups, *\({p}<0.05\) vs. CTL, #\({p}<0.05\) vs. verapamil, and&\({p}<0.05\) vs. VER.
