Figure 7
VER155008, an HSP70 inhibitor, further increases the effects of a SERCA pump inhibitor in aorta stimulated with phenylephrine. Aortic rings were challenged with phenylephrine (\(10\,\upmu \hbox {mol/l}\)) in 0[\(\hbox {Ca}^{2+}\)] Krebs’ solution in the presence of vehicle (CTL) or (A) cyclopiazonic acid (CPA) (5 and \(10\,\upmu \hbox {mol/l}\), DMSO diluted) or (B) VER155008 (\(10\,\upmu \hbox {mol/l}\), DMSO diluted) as well as the combination of CPA and VER155008. Inhibitors were added before IP3r-mediated \(\hbox {Ca}^{2+}\) release. The concentration of \(\hbox {Ca}^{2+}\) was restored after 10 min, and the force developed was evaluated for 15 min. (C) Area under the curve and (D) \(\hbox {E}_{\mathrm{max}}\). Panels (C,D) use the same color scheme as panels (A,B). Data are expressed as mean±SEM. n = 9 for CTL and VER and n = 6 for all other groups, *\({p}<0.05\) vs. CTL, #\({p}<0.05\) vs. CPA, and&\({p}<0.05\) vs. VER.
