Figure 2
From: Nrf2 regulates cell motility through RhoA–ROCK1 signalling in non-small-cell lung cancer cells
Nrf2 regulates cell motility. Nrf2 expressed was suppressed in A549 or H460 cells following treatment with brusatol (10 nM) or transfection of Keap1-Flag or siNrf2. (a,c) Horizontal migration of these cells was compared with that of the control cells using wound healing assay for the indicated time points. (b,d) Nrf2 was overexpressed by transfecting Nrf2-EGFP into the HCC827 cells. Vertical migration was detected by transwell assay after brusatol treatment or transfection for 24 h. The degree of migration was quantified by calculating the area of migrated cells using the image processing software, Image J (Ver. 1/52n, NIH, Bethesda, MD, USA). Scale bar: 200 µm *p < 0.05, **p < 0.01, ***p < 0.001 compared with control.
