Figure 3
From: Nrf2 regulates cell motility through RhoA–ROCK1 signalling in non-small-cell lung cancer cells
Molecules from the RhoA-ROCK1 pathway, and not the EMT-related molecules, are associated with Nrf2-induced cell motility. The expression levels of Nrf2, RhoA, Rock1, and/or EMT markers were detected using (a,c) western blotting or (b,d) qRT-PCR. (a,b) Nrf2 was suppressed by treating the A549 or H460 cells with brusatol (10 nM) or transfection of Keap1-Flag or siNrf2. (c,d) Nrf2 was overexpressed by transfecting HCC827 cells with Nrf2-EGFP or Keap1 mutant (D236H)-Flag for 24 h.*p < 0.05, **p < 0.01, ***p < 0.001 compared with control.
