Figure 1 | Scientific Reports

Figure 1

From: Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages

Figure 1

The levels of (a) IL-6 and (b) IL-8 in A549 cells treated with C. sativa Arbel crude and FCBD and FTHC extract fractions. Cells were treated with 300 ng/mL TNFα and C. sativa extract and fractions at a concentration of 5 μg/mL for 4 h. Dexamethasone (Dex; 4 μg/mL) served as a positive control. Control (0.5% v/v methanol) treatment served as the solvent (vehicle) control; TNFα indicates TNFα+ solvent control treatment. Error bars indicate ± standard error (sem) (n = 3). Bars labeled with different letters are significantly different from all combinations of pairs by Tukey–Kramer honest significant difference test (HSD; P ≤ 0.05). Dose–effect curves of C. sativa FCBD on (c) IL-6 and (d) IL-8 levels in the A549 cell line. Dose–effect curves of FCBD:std (93.5% CBD+ 6.1% CBG+ 0.4% THCV) on (e) IL-6 and (f) IL-8 levels in the A549 cell line. GraphPad Prism version 6.1 was used to produce the dose–response curve and IC50 doses. Error bars indicate ± sem (n = 3).

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