Table 4 Genetic diversity caculation of Orchidaceae plants based on candidate barcode sequences by the DnaSP v5 software.

Sequences	n	Nucleotide diversity					π	Neutrality tests
Sequences	n	S	k	Eta	Hd	θ	π	Fu’s Fs	p-value	D	p-value
matK	3050	1288	3.12596	1523	0.9050	0.24339	0.07270	− 2.57476	< 0.05	− 1.84286	< 0.05
rbcL	643	259	15.997	322	0.9779	0.07155	0.02503	− 0.51015	> 0.10	− 1.92689	< 0.05
ndhF	234	233	13.952	340	0.9660	0.18546	0.04589	− 2.96843	< 0.05	− 2.37565	< 0.01
ycf1	384	906	95.110	1470	0.9921	0.17379	0.07339	0.25100	> 0.10	− 1.78584	< 0.05
matK + rbcL	372	559	54.729	821	0.9924	0.12616	0.05462	− 0.11121	> 0.10	− 1.75132	< 0.05
matK + ndhF	216	687	59.444	943	0.9853	0.12276	0.04604	− 1.32301	> 0.10	− 2.00131	< 0.05
matK + ycf1	378	1495	150.984	2314	0.9932	0.15463	0.06542	0.08057	> 0.10	− 1.79023	< 0.05
ndhF + ycf1	228	494	39.916	712	0.9740	0.15585	0.05191	− 1.47948	> 0.10	− 2.13392	< 0.01

Eta Total number of mutations, n number of sequences, k Average number of nucleotide difference, S Number of segregating sites, θ nucleotide substitution rate, π nucleotide diversity, Hd haplotype diversity, Fu’s Fs is variation among different haplotypes in the population, D is the Tajima test statistic.

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