Figure 1 | Scientific Reports

Figure 1

From: A nuclear protein, PfMORC confers melatonin dependent synchrony of the human malaria parasite P. falciparum in the asexual stage

Figure 1

3D7morc-GFP construct strategy and localization. (A) Schematic representation of the 3D7morc-GFP integration strategy and gene after the integration; (B) diagnostic PCR; (C) Western blot of P. falciparum transfected with 3D7morc-GFP clones with wild type P. falciparum (3D7) showing no band (~ 300 kDa) with anti-GFP antibody, and (D) confocal microscopy was performed to localize PfMORC protein in 3D7morc-GFP-infected erythrocytes. Asexual parasites at ring (6 h.p.i.), early-trophozoite (24 h.p.i.), late-trophozoite (34 h.p.i.) and schizont (44 h.p.i.) stage were used to localize the PfMORC. Parasites were also stained with the nuclear stain DAPI. The colocalization of GFP with the DAPI signal confirms that PfMORC localizes in the nucleus of the asexual developmental stages.

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