Figure 2
Real-time expression of PfMORC. (A) Reverse transcription real-time PCR (RT-qPCR) of PfMORC expression in different stages of 3D7 P. falciparum parasites. Stages—Ring (~ 10 h), Trophozoites (~ 30 h) and Schizonts (~ 42 h). (B) Validation by RT-qPCR of PfMORC expression in trophozoites stage (30 h post-infection) of 3D7; (C) PfPK7− and (C) PfPK7 complement P. falciparum parasites. Trophozoites stage parasites were treated with 100 nM of melatonin for a period of 5 or 17 h. (D) Investigation of PfMORC expression pattern by RT-qPCR in 3D7 P. falciparum parasites. Ring stage parasites (6 h) were treated with 100 nM melatonin for a period of 24 h; the sample was collected every hour for the next 24 h period. The results were plotted as fold change in 1 h difference to the first collection. First, the relative expression was normalized with the reference gene (PF3D7_0717700) to obtain ΔCt value and then with ΔCt of 1 h control and melatonin treatment, respectively to obtain ΔΔCt to calculate fold change in PfMORC expression. All the experiments were performed three times in triplicate and results are shown as mean ± SD. Statistical significance was performed by student t-test. *< 0.05.
