Figure 3 | Scientific Reports

Figure 3

From: A nuclear protein, PfMORC confers melatonin dependent synchrony of the human malaria parasite P. falciparum in the asexual stage

Figure 3

3D7morc-glmS construct strategy and localization. (A) Schematic representation of the 3D7morc-glmS integration strategy and gene after the integration; (B) Western blot of 3D7morc-glmS parasites at different asexual stages showing different protein expression and wild type P. falciparum (3D7) as negative control showing no band (~ 300KDa) and (C) immunofluorescence assay and confocal microscopy to localize PfMORC in P. falciparum-infected erythrocytes (Ring, Trophozoite and Schizont Stage). Co-localization with nucleus stain DAPI confirms the GFP imaging that PfMORC localizes in the nucleus.

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