Figure 4
Effect of glucosamine treatment in conditional knockdown of PfMORC parasites. (A) Western blot analysis of 3D7morc-glmS parasites treated with 0–2.5 mM of glucosamine for 48 h showing reduced protein expression, but the control 3D7morc-M9 parasites did not exhibit down regulation in protein level. (B) Early trophozoites were treated with and without 2.5 mM glucosamine till the next cycle and smears were made when majority of parasites reached to segmented schizont. The average of merozoites per schizont in populations was obtained by counting at least 50 parasites for each replicate. (C) Parasitemia was monitored for 6 days in the presence of a different concentration of glucosamine in the media for 3D7morc-glmS and (D) 3D7morc-M9. No significant statistical difference was observed when unpaired t-test was performed.
