Figure 5
Effect of melatonin after knocking down PfMORC protein expression. Asynchronous parasites were first treated with or without 2.5 mM glucosamine for 48 h and then incubated with and without melatonin (100 nM) for 24 h. The parasites were stained with nuclear dye YOYO-1 and passed through the flow cytometer. R + T and S forms of the parasite population were calculated and plotted the percent population for both 3D7morc-M9 and 3D7morc-glmS. (A) Absence of melatonin shows high mono nucleated R + T form but 100 nM melatonin treatment increases the mature stage parasites in 3D7morc-M9 irrespective of glucosamine treatment; (B) but the effect of melatonin was partly interrupted to increase the S form 3D7morc-glmS, which is sensitive to glucosamine treatment, albeit R + T remained relatively similar to control. (C,D) and (E,F) represent the dot plot representation for melatonin effect with and without glucosamine for 3D7morc-M9 and 3D7morc-glmS, respectively. Each graphics represents the three independent experiments in triplicate for mean ± SD and the statistical difference was obtained by one-way ANOVA using Dunn’s multiple comparison test. *> 0.05, **> 0.01, ***> 0.001.
