Figure 1
Genetic identity of the phased Pelophylax genomes based on 81 individuals and five families. RR denotes P. ridibundus mothers and daughters, RL denotes P. esculentus fathers and sons. R denotes sexual genomes, [R] and [L] denote clonal genomes. (A) An illustration of the laboratory crossing experiments that resulted in R[R] daughters and R[L] sons; photos show real individuals participating in the experiment; * late prophase I of the P. esculentus male´s first meiotic division using comparative genomic in-situ hybridization with lessonae specific probes (red color) and ridibundus specific probes (green color); see Doležálková et al.34. (B1) fineRADstucture heatmap of haplotypes similarity (2219 SNPs): co-ancestry matrix based on a RADseq dataset, above the diagonal are the individual scores, below are the population averages. The color scale legend on the right indicates the relatedness between haploid genomes. A cluster of clonal [L] genomes is apparent in the upper right of the graph (black/dark blue color), while sexual R and clonal [R] on the down left (rose/red color). (B2) STRUCTURE ancestry bar plots of the respective individuals, Q-value scale indicated on the left. Phased haploid genomes of mothers are indicated with ♀ and fathers with ♂, other genomes representing progeny with families´ membership indicated with frames. (C) Principal component analysis (PCA) of phased microsatellite data based on 15 loci. (D) Results of PCA based on phased genotypes of ddRADseq data (2219 SNPs). The clusters determine sexual R/clonal [R] genomes and clonal [L] genomes. Each point represents an individual haploid multilocus genotype; one color and symbol denote one family.
