Figure 1
Porcine LFA-1 confers susceptibility to ApxI in HEK 293T cells. (a) HEK 293T cells were transfected with plasmids expressing porcine CD11a (pCD11a) and/or porcine CD18 (pCD18) or the corresponding empty vector. Ectopic expression of pCD11a and pCD18 was examined using confocal microscopy. Confocal images show the subcellular localization of pCD11a (pseudogreen), pCD18 (pseudored), and nuclei (pseudoblue). (b) Cells were transfected with plasmids expressing pCD11a and pCD18, green fluorescent protein (GFP), or non-transfected (None) for 24 h, followed by incubation with 1.6–25 CU/ml of ApxI for 5 h. The culture supernatants were collected and analyzed using the LDH release assay. Data are from three independent experiments of triplicate determinants. Error bars represent the standard deviations. *p < 0.05; **p < 0.01; ***p < 0.001. (c) Cells were transfected with plasmids expressing pCD11a and pCD18 or GFP. Expression of pCD11a and pCD18 was examined using Western blot analysis. Immunoblots were also probed with an anti-β-actin antibody as the loading control. The grouping of blots was cropped from different portions of the same gel and exposed separately. Uncropped blot images for (c) are presented in Supplementary Figure S1.
